PichiaWorks

PichiaWorks

$1,200.00 USD

Ginkgo’s PichiaWorks Pichia pastoris Protein Expression Kits deliver high-level recombinant protein production without the usual headaches.

  • Methanol-Free: Glucose-depletion induction completely eliminates the need for hazardous methanol.
  • Rapid, Reliable Screening: A site-specific integration system ensures consistent, high-level protein expression from day one, drastically reducing your screening time.

Ginkgo’s PichiaWorks Kit is the workhorse of the PichiaWorks lineup. Powered by Ginkgo’s robust base strain, this kit delivers dependable, high-level protein expression right out of the box. The kit contains all required items to build five highly expressing Pichia strains.

Contents

  • PichiaWorks Strain1 competent cells
  • Vector for cytosolic protein expression 
  • Vector for secreted protein expression 
  • Control vector for expression of cytosolic mCherry

  • Amplification primers

PichiaWorks Handbook

FAQ

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Terms of Use

SDS: US, EU

PichiaWorks Protein Expression kits are for research use only. Administration to human and other animals and use in the clinic, for diagnostic purposes, and other uses are prohibited. Do not use in foods or for domestic purposes.
PichiaWorks
  • PichiaWorks
  • PichiaWorks

We evaluated mCherry expression in Pichia under three promoters over a 72-hour flask fermentation: pGAP (constitutive), pAOX1 (methanol-inducible), and pSynPro (proprietary PichiaWorks strain specific). All strains were genetically engineered with a single-copy expression cassette at the same chromosomal locus.

Higher protein levels and easier protocols than standard strains

  • Nearly twice the protein production levels of leading systems
  • Safe inducible protocol - no methanol needed!
  • Simplified culturing protocol - no pellet washing before induction required 
  • Clear path to scale-up