Ginkgo’s CFPS Ready384 consists of Ginkgo’s Premium CFPS system, reformatted to meet high-throughput screening needs. Reactions are pre-mixed and aliquoted into a 384-well plate. The user must simply add DNA and water, and reactions will produce target protein within hours.
The kit is an E. coli transcription/translation system optimized to produce proteins from a T7 RNA Polymerase promoter. Reactions consist of a bacterial cell lysate and a reagent mix that contain all components required for protein production.
Each plate enables 352 reactions of 10 µL. Of this volume, 6 µL are pre-aliquoted, and the user supplies the remaining 4 µL of DNA, water, and any other desired reagents. Columns 23 and 24 are left empty for customer-specified controls or standards. The plate is black with a clear bottom to enable in situ fluorescence and absorbance measurements.
This kit is compatible with the Solubility Enhancer (sold separately).
Contents
CFPS User Manual
CFPS Ready384 Quick Start Guide
Control Plasmid Sequence
Terms of Use
Safety Data Sheet: US
Cell-Free Protein Synthesis kits are for research use only. Administration to human and other animals and use in the clinic, for diagnostic purposes, and other uses are prohibited. Do not use in foods or for domestic purposes.
Do you have questions about our kit or want a consult on how to best use Ginkgo's CFPS kits? Are you interested in a custom or bulk order? Contact us