PichiaWorks KO

Ginkgo’s PichiaWorks Pichia pastoris Protein Expression Kits deliver high-level recombinant protein production without the usual headaches.

• Methanol-Free: Glucose-depletion induction completely eliminates the need for hazardous methanol.
• Rapid, Reliable Screening: A site-specific integration system ensures consistent, high-level protein expression from day one, drastically reducing your screening time.

Ginkgo’s PichiaWorks KO kit features specific engineered knockout strains from the KO Panel, allowing you to lock in the ideal protease profile and maximize your protein's expression levels. The kit contains all required items to build five highly expressing Pichia strains.

Contents:

• PichiaWorks StrainKO competent cells (genotype specific to product)
  • Strain KO1: Δpep4, Δysp3
  • Strain KO2: Δpep4, Δyps1-1
  • Strain KO3: Δpep4, Δkex1
  • Strain KO4: Δpep4, Δprc1
  • Strain KO5: Δpep4, Δpas_chr1-1_0379
• Vector for cytosolic protein expression 
• Vector for secreted protein expression 
• Control vector for expression of cytosolic mCherry

• Amplification primers

PichiaWorks Handbook

FAQ

Do you have questions about our kit or want a consult on how to best use PichiaWorks? Contact us here and we'll connect you with the appropriate expert.

Terms of Use

SDS: US, EU

PichiaWorks Protein Expression kits are for research use only. Administration to human and other animals and use in the clinic, for diagnostic purposes, and other uses are prohibited. Do not use in foods or for domestic purposes.