PichiaWorks Protein Expression System

PichiaWorks Protein Expression System

$1,200.00 USD

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Ginkgo’s PichiaWorks Protein Expression Kit is a Pichia pastoris based system designed for  high-level production of cytosolic and secreted recombinant proteins. The kit provides sufficient material to build five Pichia strains. 

PichiaWorks builds on all of the advantages of P. pastoris, but notably does not use methanol for induction. Instead, it has been engineered for high expression with just a simple glucose-induction protocol.  The kit contains all required items for building highly expressing Pichia strains: competent cells with landing pads for your gene of interest and the molecular machinery that enables high-efficiency integration.  

Contents

  • PichiaWorks Strain1 competent cells
  • Vector for cytosolic protein expression 
  • Vector for secreted protein expression 
  • Control vector for expression of cytosolic mCherry

  • Amplification primers

Expression Handbook

Do you have questions about our kit or want a consult on how to best use PichiaWorks? Email us at reagents@ginkgo.bio, and we'll connect you with the appropriate expert.

Terms of Use

PichiaWorks Protein Expression kits are for research use only. Administration to human and other animals and use in the clinic, for diagnostic purposes, and other uses are prohibited. Do not use in foods or for domestic purposes.

 
PichiaWorks Protein Expression System
  • PichiaWorks Protein Expression System
  • PichiaWorks Protein Expression System

We evaluated mCherry expression in Pichia under three promoters over a 72-hour flask fermentation: pGAP (constitutive), pAOX1 (methanol-inducible), and pSynPro (proprietary PichiaWorks strain specific). All strains were genetically engineered with a single-copy expression cassette at the same chromosomal locus.

The PichiaWorks strain significantly outperformed traditional systems, showing higher fluorescence by 48 hours and achieving maximum intensity at 72 hours. Unlike the labor-intensive methanol induction, which requires biomass pelleting and resuspension, the PichiaWorks system simplifies the process, achieving higher protein yields by a simple 2% v/v preculture inoculation into the production media.

Higher protein levels and easier protocols than standard strains

  • Nearly twice the protein production levels of leading systems
  • Safe inducible protocol - no methanol needed!
  • Simplified culturing protocol - no pellet washing before induction required 
  • Clear path to scale-up