Ginkgo’s cell free protein synthesis (CFPS) kit is an E. coli transcription/translation system optimized to produce proteins from a T7 RNA Polymerase promoter. Reactions consist of a bacterial cell lysate and a reagent mix that contain all components required for protein production; the user must simply provide a DNA template that encodes the protein of interest under control of a T7 promoter. The system is easy-to-use, produces protein within a few hours, and is compatible with high-throughput liquid handling and automation platforms. Protein yield and function can be evaluated in solution or following purification.
10 mL kit enables user to run 1000 reactions of 10 µL, 200 reactions of 50 µL, or user can scale reaction volumes to their own specifications
100 mL kit enables user to run 10,000 reactions of 10 µL, 2000 reactions of 50 µL, or user can scale reaction volumes to their own specifications
Contents
Quick Start Protocol and Construct Design
Do you have questions about our kit or want a consult on how to best use Ginkgo's CFPS kits? Email us at reagents@ginkgo.bio, and we'll connect you with the appropriate expert.
Cell-Free Protein Synthesis kits are for research use only. Administration to human and other animals and use in the clinic, for diagnostic purposes, and other uses are prohibited. Do not use in foods or for domestic purposes.
Interested in a custom order? Reach out to us at reagents@ginkgo.bio
