DSB Booster - Beta release

DSB Booster - Beta release

$140.00 USD
Volume

The DSB Booster (20x) is an optimized formula designed to enable the formation of disulfide bonds during cell-free protein synthesis. It establishes the redox environment your target protein needs to pair its cysteines correctly, so that more of what you synthesize comes out of the reaction correctly folded and functional.

This 20x concentrated solution is included as a part of Ginkgo's CFPS Disulfide Premium kit, and can be purchased separately for users who want to titrate beyond the amount supplied in the kit.

Contents:

  • DSB Booster (20x)

Technical Information:

  • The standard dose is 1 µL per 20 µL reaction (1x, 5% v/v).
  • Available sizes are 50 μL, which supports 50 reactions at 1x, and 500 μL, which supports 500 reactions at 1x.
  • Thaw on ice before use and minimize freeze-thaw cycles.
  • Store at −80°C.
  • The product ships on dry ice.

CFPS Disulfide User Manual

Terms of Use

Safety Data Sheet 

Cell-Free Protein Synthesis kits are for research use only. Administration to human and other animals and use in the clinic, for diagnostic purposes, and other uses are prohibited. Do not use in foods or for domestic purposes.

Do you have questions about our kit or want a consult on how to best use Ginkgo's CFPS kits? Are you interested in a custom order? Reach out to us at [email protected]
DSB Booster - Beta release
  • DSB Booster - Beta release
  • DSB Booster - Beta release

DSB Booster dose response scales with disulfide-bond complexity. Relative yield of three model targets spanning a range of disulfide-bond content (VHH, 1 to 2 disulfides; Gaussia luciferase, 5 disulfides; reteplase, 9 disulfides) as a function of DSB Booster dose. Values are normalized within each target to the yield obtained at the standard 1x booster dose (solid bars). Hatched segments represent the incremental yield delivered by increasing booster dose above 1x, with labels giving the percentage gain relative to 1x. Error bars show standard deviation of replicate reactions (n = 3)

DSB Booster increases oxidative folding in a dose-dependent manner

  • The DSB Booster is key for proper formation of disulfide bonds
  • 1x dose delivers substantial signal for targets containing up to 9 disulfide bonds
  • The tougher proteins benefit from DSB Booster titration: a real lever for your hardest constructs.
  • One variable to tune. Nothing else in the reaction changes.