{"product_id":"cfps-dsb-premium","title":"CFPS Disulfide Premium - Beta release","description":"\u003cp dir=\"ltr\"\u003e\u003cspan\u003eGinkgo’s CFPS Disulfide Premium kit is tailored for the production of proteins containing disulfide bonds. It enables the synthesis of functional disulfide bonded targets that can’t properly express with standard CFPS, including antibody fragments, multi-domain therapeutic enzymes, and more.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp dir=\"ltr\"\u003e\u003cspan\u003eThe kit is an \u003c\/span\u003e\u003cem\u003eE. coli\u003c\/em\u003e\u003cspan\u003e transcription\/translation system optimized to produce proteins from a T7 RNA Polymerase promoter under redox conditions that facilitate disulfide bond (DSB) formation. Reactions consist of a bacterial cell lysate, a reagent mix containing all components required for protein production, and a DSB Booster that drives formation and correct pairing of DSBs in the target protein. The user must simply provide a DNA template that encodes the protein of interest under control of a T7 promoter. Reactions are assembled on ice, incubated at 30°C for 4 - 18 hours, and can be analyzed in solution or after purification.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp dir=\"ltr\"\u003e\u003cspan\u003eThe system has been optimized for proteins carrying 1 to 9 disulfide bonds and validated across protein classes spanning that range:\u003c\/span\u003e\u003c\/p\u003e\n\u003cul\u003e\n\u003cli dir=\"ltr\" role=\"presentation\"\u003e\u003cspan\u003eAntibody fragments: VHHs and scFvs (1–2 disulfide bonds), expressed with \u0026gt;95% DSB confirmed via intact mass spectrometry)\u003c\/span\u003e\u003c\/li\u003e\n\u003cli dir=\"ltr\" role=\"presentation\"\u003e\n\u003cspan\u003eReporter enzymes: \u003c\/span\u003e\u003cspan\u003eGaussia\u003c\/span\u003e\u003cspan\u003e luciferase (5 disulfide bonds), supplied as the kit's positive control\u003c\/span\u003e\n\u003c\/li\u003e\n\u003cli dir=\"ltr\" role=\"presentation\"\u003e\n\u003cspan\u003eMulti-domain therapeutic enzymes: reteplase (9 disulfide bonds), a plasminogen activator that is notoriously difficult to express solubly in conventional \u003c\/span\u003e\u003cspan\u003eE. coli\u003c\/span\u003e\u003cspan\u003e systems\u003c\/span\u003e\n\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cp\u003e\u003cspan\u003e\u003cb id=\"docs-internal-guid-eefff3ec-7fff-6390-38c5-6400fe128706\"\u003e\u003c\/b\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp dir=\"ltr\"\u003e\u003cspan\u003eContents:\u003c\/span\u003e\u003c\/p\u003e\n\u003cul\u003e\n\u003cli dir=\"ltr\"\u003e\n\u003cspan\u003eDSB \u003c\/span\u003e\u003cspan\u003eE. coli\u003c\/span\u003e\u003cspan\u003e Lysate\u003c\/span\u003e\n\u003c\/li\u003e\n\u003cli dir=\"ltr\"\u003e\u003cspan\u003eDSB Reagent Mix (3.3x)\u003c\/span\u003e\u003c\/li\u003e\n\u003cli dir=\"ltr\"\u003e\u003cspan\u003eDSB Booster (20x)\u003c\/span\u003e\u003c\/li\u003e\n\u003cli dir=\"ltr\"\u003e\u003cspan\u003eControl \u003cmeta charset=\"utf-8\"\u003eGaussia Luciferase (GLuc) Plasmid (100 nM)\u003c\/span\u003e\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cp dir=\"ltr\"\u003e\u003cspan\u003eTechnical Information:\u003cbr\u003e\u003cmeta charset=\"utf-8\"\u003e \u003cmeta charset=\"utf-8\"\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cul\u003e\n\u003cli dir=\"ltr\" role=\"presentation\"\u003e\u003cspan\u003eOptimized for targets containing 1 to 9 disulfide bonds.\u003c\/span\u003e\u003c\/li\u003e\n\u003cli dir=\"ltr\" role=\"presentation\"\u003e\u003cspan\u003eCompatible with both plasmid DNA and linear DNA templates. Eliminate time-consuming cloning steps by using linear DNA directly.\u003c\/span\u003e\u003c\/li\u003e\n\u003cli dir=\"ltr\" role=\"presentation\"\u003e\u003cspan\u003eExpression is driven by a T7 RNA polymerase promoter.\u003c\/span\u003e\u003c\/li\u003e\n\u003cli dir=\"ltr\" role=\"presentation\"\u003e\u003cspan\u003eReaction volumes can be scaled up or down proportionally from the standard recommended 20 uL setup.\u003c\/span\u003e\u003c\/li\u003e\n\u003cli dir=\"ltr\" role=\"presentation\"\u003e\n\u003cspan\u003eIncludes a \u003c\/span\u003e\u003cspan\u003eGaussia\u003c\/span\u003e\u003cspan\u003e luciferase (Gluc) positive control to verify successful CFPS.\u003c\/span\u003e\n\u003c\/li\u003e\n\u003cli dir=\"ltr\" role=\"presentation\"\u003e\u003cspan\u003eIncubation at 30 °C for 4 to 18 hours is ideal across various protein classes (although target-specific optimization may improve titers).\u003c\/span\u003e\u003c\/li\u003e\n\u003cli dir=\"ltr\" role=\"presentation\"\u003e\u003cspan\u003eKit is stored at −80 °C. GLuc Control Plasmid at −20 °C.\u003c\/span\u003e\u003c\/li\u003e\n\u003cli dir=\"ltr\" role=\"presentation\"\u003e\u003cspan\u003eThe kit ships on dry ice.\u003c\/span\u003e\u003c\/li\u003e\n\u003cli dir=\"ltr\" role=\"presentation\"\u003e\u003cspan\u003eThis beta release is not compatible with downstream purification via His-tag (IMAC\/Ni-NTA) affinity. Use an alternative affinity tag such as FLAG or Strep-II, or recover protein by size-exclusion or ion-exchange chromatography.\u003c\/span\u003e\u003c\/li\u003e\n\u003cli dir=\"ltr\" role=\"presentation\"\u003e\u003cspan\u003eThis kit is for research use only and is not for use in diagnostic or therapeutic procedures.\u003c\/span\u003e\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cp\u003e\u003cb id=\"docs-internal-guid-e2356c65-7fff-0150-94ed-561c198fd2bb\"\u003e\u003c\/b\u003e\u003c\/p\u003e\n\u003cp\u003e\u003ca href=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0759\/9816\/7283\/files\/CFPS_Disulfide_Premium_Quick_Start.pdf\" title=\"CFPS Premium Quick Start Guide\" rel=\"noopener\" target=\"_blank\"\u003eQuick Start Guide\u003c\/a\u003e\u003c\/p\u003e\n\u003cp\u003e\u003ca href=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0759\/9816\/7283\/files\/cfps-dsb-user-manual.pdf\" title=\"CFPS Disulfide User Manual\" rel=\"noopener\" target=\"_blank\"\u003eCFPS Disulfide User Manual\u003c\/a\u003e\u003c\/p\u003e\n\u003cp\u003e\u003ca href=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0759\/9816\/7283\/files\/cell-free-lysate-terms-of-use.pdf?v=1748034302\" title=\"Terms of Use\" rel=\"noopener\" target=\"_blank\"\u003eTerms of Use\u003c\/a\u003e\u003c\/p\u003e\n\u003cp\u003eSafety Data Sheet\u003c\/p\u003e\n\u003cp\u003eCell-Free Protein Synthesis kits are for research use only. Administration to human and other animals and use in the clinic, for diagnostic purposes, and other uses are prohibited. Do not use in foods or for domestic purposes.\u003c\/p\u003e\n\u003cp\u003eDo you have questions about our kit or want a consult on how to best use Ginkgo's CFPS kits? Are you interested in a custom order? Reach out to us at reagents@ginkgo.bio\u003c\/p\u003e","brand":"Ginkgo Reagents","offers":[{"title":"1 mL","offer_id":50532420223219,"sku":"CF021.1","price":575.0,"currency_code":"USD","in_stock":true},{"title":"10 mL","offer_id":50532420255987,"sku":"CF022.1","price":3900.0,"currency_code":"USD","in_stock":true},{"title":"100 mL","offer_id":50532420288755,"sku":"CF023.1","price":35000.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0759\/9816\/7283\/files\/cfps-dsb-green-clear_ea189502-c345-42fd-b469-505e8deb2b4d.png?v=1789074350","url":"https:\/\/reagents.ginkgo.bio\/products\/cfps-dsb-premium","provider":"Ginkgo Reagents","version":"1.0","type":"link"}